An online resource for quality-checked nucleotide sequences of myxomycetes linked to herbarium specimens of fruit bodies (sporocarps) with reliable taxonomic determination and accompanied with metadata (photographs of morphological structures of reference specimens, sequence chromatograms, specimen collection data, etc.). The aim of MyxoSeq is to provide a curated collection of reference sequences, with the first part of the 18S rRNA gene accepted as the main DNA barcode for the group, which can be used for taxonomic annotation of the specimens of sporocarps, living cultures, and environmental sequences. Taxonomic annotation of sequences is checked based on specimen morphology, position in molecular phylogeny, and similarity to other sequences.
Anastasia Vlasenko, Andrey Matveev, Dmitry Leontyev, Fedor Bortnikov, Ilya Prikhodko, Inna Zemlyanskaya, Martin Schnittler, Nadezhda Fedorova, Nikki Heherson Dagamac, Oleg Shchepin, Vladimir Gmoshinskiy, Yuri Novozhilov.
Komarov Botanical Institute, Russian Academy of Sciences, Saint Petersburg, Russia
Lomonosov Moscow State University, Russia
Central Siberian Botanical Garden of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
Institute of Botany and Landscape Ecology, Greifswald University, Germany
This information system was created under the project “DNA barcoding of myxomycetes (Myxomycetes = Myxogastria) and analysis of their hidden diversity based on herbarium collections and metagenomic data” (18-04-01232 А) funded by the Russian Foundation of Basic Research, and in framework of two programs of the Komarov Botanical Institute: “Taxonomic diversity, ecology and physiological and biochemical features of fungi and fungus-like protists of Vietnam” (AAAA-A19-119080990059-1) and “Biodiversity, ecology, structural and functional features of fungi and fungus-like protists” (АААА-А19-119020890079-6). Additional support was provided by the “RESPONSE” project of the German Research Council (DFG: SCHN1080/2-1, RTG 2010).
We are grateful to all researchers who provided specimens of sporocarps of myxomycetes for DNA extraction and sequencing.
Administrator: Oleg Shchepin, e-mail: OShchepin@binran.ru
Developer: Andrey Matveev, e-mail: AMatveev@binran.ru